After the solution is effected, add sufficient water to produce 100 ml. Add 0.1 ml of 0.1 M eerie ammonium nitrate; the color changes from redto light blue. It is recommended to experiment with a 5 mL aliquot to get an idea of the color and titre. How To Choose A Digital Camera Of Your Choice? it melts with decomposition at a temperature above 300. Stability: :Stable. Photometers, See Full Listing of
Stability: :Stable. Acids & Bases; Buffers; Dyes, Stains, Indicators; . If necessary, adjust the pH of the mixture to 4.7. Conductivity Measurement, See Full Listing of
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Not more than 0.05 ml of 0.1 M perchloric acid is required to change the color of the solution from brownish yellow to green. xref
Stir. Separating, Centrifuging, ENVIRONMENTAL - Soil, Water and Food Analysis, See Full Listing of
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The complexing agent binds to the metals and, after all the metals have been complexed, the next drop of complexing agents binds to an indicator to induce a color change. General Laboratory Reagents, See Full Listing of
Add only very small quantities of the indicator are needed. Refractive Index Standards, See Full Listing of
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The indicator is stable for at least 12 months when stored in a polythene bottle and in the dark. Eriochrome black T indicator: Mix 0.5 g Eriochrome black T dye with 4.5 g hydroxylamine hydrochloride. Dissolve 150 mg of methylene blue in 100 ml of ethanol (95 percent) and dilute with ethanol (95 percent) to produce 250 ml. Hygiene, See Full Listing of
Partly soluble in water and in alcohol. Eriochrome Black T Indicator Mixture; Eriochrome Black T Triturate; Mordant Black 11 Mixture: A mixture of 1 part of eriochrome black T and 99 parts of sodium chloride. These cookies will be stored in your browser only with your consent. Tools, See Full Listing of
Let stand for a sufficient length of time, so that all bubbles disperse. Interview Questions and Answers hbbe`b``3i~0
of their rangewith the corresponding color changes. 0000002921 00000 n
After removal of the plate, allow it to dry in air. After the solution is effected, add sufficient water to produce 100 ml. Colour changes from blue to red (pH range, 9.0 to 13.0). Put on gloves and protective eyewear and weigh out approximately 0.5 g of solid Eriochrome Black T, (EBT) on a balance and transfer it to a small beaker or flask. Skin Protection, See Full Listing of
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Dissolve 0.1 g of thymol blue in a mixture of 2.2 ml of 0.1 M sodium hydroxide and 50 ml of ethanol (95 percent) and dilute to 100 ml with water. The cookie is used to store the user consent for the cookies in the category "Performance". and 2-3 drops of Eriochrome Black T indicator solution. xref
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We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. Bromothymol Blue Indicator; 4,4' -(3H-2, I-Benzoxathiol-3-ylidene)bis(, Dissolve 50 mg of bromothymol blue in 4 ml of 0.02. Required fields are marked *. Then calculate an average percentage. Brownish green, crystalline powder; soluble in ethanol (95 percent) and in dilute alkali solutions; slightly soluble in water. Cultivation, See Full Listing of
All wastes must be handled in accordance with local, state, and federal regulations. Dissolve 50 mg of methyl red in a mixture of 1.86 ml of 0.1 M sodium hydroxide and 50 ml of ethanol (95 percent). Ultrasonic Cleaners, See Full Listing of
ICH Q3D Standards (USP 232/233/2232, EP 520, ChP, JP, Indian Pharmacopoeias), See Full Listing of
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Then accurately weigh out about .95 g 0.lmg. 0000008621 00000 n
How must i study physics for that IIT preparation? Titrate with 0.01 M EDTA until the solution turns from wine red to sky blue with no hint of red (save the solution for colour comparison). Dry about 2 g of EDTA dihydrate, Na2H2Y2 2H2O, in a drying oven at 80C for one hour. SENSITIVITY - A mixture of 0.1 ml of the solution, 100 ml of carbon dioxide-free water and 0.05 ml of 0.02 M hydrochloric acid is red. (TAN) / Total Base No. Analytical cookies are used to understand how visitors interact with the website. Reagecon's Eriochrome Black T Indicator 1% (w/w) in Sodium Chloride is a high quality Indicator produced from the highest quality raw materials and manufactured and tested under rigorous conditions. Methyl Red- Methylene Blue Indicator Solution; Methyl Red, Dissolve 0.1 g of methyl red and 50 mg of methylene. Dissolve 0.1 g of thymol blue in 2.15 ml of 0.1 M sodium hydroxide and 20 ml of ethanol (95 percent). SENSITIVITY - A mixture of 0.1 m] of the solution and 100 ml of carbon dioxide-free water is yellow. The sample is decomposed with hydroxylamine hydrochloride solution. 6 How do you make a Ferroin indicator solution? When metal ions are absent, for example, in the presence of an excess of disodium edetate, the solution is grey. Not more than 0.1 5 ml of 0.02 M hydrochloric acid is required to change the color to yellow. The four hydrogens in the formula refer to the four acidic hydrogens on the four carboxyl groups. Digest the extracted litmus with 250 ml of water and filter. water to which 0.2 ml of0.02 M sodium hydroxide has been added is blue. Advertisement cookies are used to provide visitors with relevant ads and marketing campaigns. Pipetting, See Full Listing of
If foaming occurs it can be suppressed by the addition of 1 or 2 drops of n-octanol. Oxygen Measurement, See Full Listing of
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When the EDTA has chelated all the Mg+2present in solution, the indicator (free and uncomplexed to Mg+2) will be robins egg blue. 1 How do you make an eriochrome black T indicator solution? In that case add 0.0100 M EDTA solution dropwise until the solution just turns blue. Pack Qty. Sterilisation, See Full Listing of
0000009473 00000 n
Subtotal: 0.00 Order No. The accuracy of these titrations is high and they offer the possibility of determinations of metal ions at concentrations at the millimole level. SENSITIVITY - A mixture of 0.1 ml of the solution and 100 ml of carbon. ! HOMOGENEITY Carry out the method for thin-layer chromatography (2. Reagecon Eriochrome Black T Indicator 1% (w/w) in Sodium Chloride. Your email address will not be published. Functional cookies help to perform certain functionalities like sharing the content of the website on social media platforms, collect feedbacks, and other third-party features. Melting Point Standards, See Full Listing of
Brilliant Green; CI 42040; Malachite Green G; Basic Green 1 Indicator: A 0.5 per cent w/v solution of brilliant green in glacial acetic acid. Why can Eriochrome Black T not be used directly as an indicator? Volumetry, See Full Listing of
While it doesn't appear to dissolve in ethanol, EBT will turn it red if added. 0000038759 00000 n
Eriochrome Black T Indicator. Desiccators, See Full Listing of
This cookie is set by GDPR Cookie Consent plugin. From your experimental data calculate the percentage of Ca in the powdered milk for each aliquot that you titrated. Titrate with the standard 0.0100 M EDTA solution to a color change from red to blue. Performance Grade Solvents, See Full Listing of
2. What are the conditional constants for Mg2+ and Ca2+ at the pH at which the titration is carried out? Redox Standards, See Full Listing of
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7mKy3c d(jwF`Mt?0wKY{jGO.AW,eU"^0E: ~"G vPKD"(N1PzbtN]716.^`[ Eriochrome Black T indicator is available in 100g containers in our online shop, where you can also get discounts on bulk orders. Acetaldehyde TS Mix 4 mL of acetaldehyde, 3 mL of alcohol, and 1 mL of water. While it doesn't appear to dissolve in ethanol, EBT will turn it red if added. endstream
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Add 2 mL of pH 10 buffer solution and add a few drops of Eriochrome Black T indicator solution. Add enough 95 percent ethyl alcohol to bring the total volume to exactly 100 mL. Gloves & Finger Cots, See Full Listing of
At the equivalence point and beyond, pCa is determined from the dissociation of the chelate at the given pH. When used for titrations in non-aqueous media, changes from violet (basic) through blue-green (neutral) to yellowish green (acidic). Add 0.1 ml of 0.1 M perchloric acid; the solution turns blue-green. 0000000016 00000 n
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Adjust the pH of the solution to 5.3 0.1, if necessary (Solution A). Yellow crystalline leaflets; mp, about I16. Sample Analysis, See Full Listing of
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So, when all metal ions are bound to EDTA, indicator EBT remains free in the sample and the solution turns blue. 4! Diaphragm Pumps, See Full Listing of
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to change the color from yellow to violet red. SENSITIVITY - Add 0.25 ml to 50 ml of anhydrous glacial acetic acid. However, you may visit "Cookie Settings" to provide a controlled consent. pH Buffer Solutions, See Full Listing of
EBT, however, is a solid and must be prepared as a solution prior to its use as an indicator. Dark red or reddish brown powder. If the solution is red, Mg 2+ is in excess. Recycling Versus. Blue, ethanol-soluble dye used as a screening agent in methyl orange-xylene cyanol FF solution. Hydroxylamine hydrochloride is highly toxic and corrosive to skin and mucous membranes. Analytical Volumetric Solutions & Indicators, ICP, AAS, IC and Flame Photometry Standards, See Full Listing of
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ScienceBriefss a new way to stay up to date with the latest science news! using Eriochrome Black T indicator to determine, by difference, the concentration of Mg2+ ions in the sample. Analytical Microbiology, See Full Listing of
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What is the purpose of adding NaOH solution dropwise to the Mg-EDTA mixture? Can be strongly diluted and poured down the drain. Flame Photometry Standards, See Full Listing of
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Transfer quantitatively with repeated washings with distilled water into a 250 mL volumetric flask. EBT, however, is a solid and must be prepared as a solution prior to its use as an indicator. Eriochrome Black T (C.I. JHUJLRKQZAPSDP-GXTSIBQPSA-M. Synonym. Microfluidics, See Full Listing of
Preparation of 0.5% (w/w) Eriochrome black T (EBT) Indicator solution. Analytical Volumetric Solutions, See Full Listing of
Eriochrome Black T Indicator Solution; Mordant Black II Indicator Solution: Dissolve 0.2 g of eriochrome black T and 2 g of hydroxylamine hydrochloride in sufficient methanol to produce 50 ml. of 50 ml of water, 1 ml of 2 M acetic acid and 0.05 ml of leadnitrate solution. Eriochrome BlackT indicator Take 100 ml of ethanol and dissolve 4.5 g hydroxylamine hydrochloridein it. in the water hardness determination process. Add about 50 mL of 95 percent ethyl alcohol and swirl the mixture until the EBT has fully dissolved. These cookies track visitors across websites and collect information to provide customized ads. A 0.1 percent w/v solution of quinaldine red in methanol. Orange-yellow powder or crystalline scales; Sparingly soluble in hot water; slightly soluble in water; practically insoluble in ethanol. Add 0.1 ml of 0.1M disodium edetate; the color changes to yellow. Transport, See Full Listing of
Pipet 20.00 mL MgCO3 standard solution into a 250 mL conical flask, and add 10 mL NH3-NH4Cl buffer solution of pH 10 and 2~3 drops Eriochrome black T indicator. HWM6W- ~jgvuR(J0$FC*$8c HJ9b\I_~wfLJlduPl Buffer solution (boric acid/potassium chloride/sodium hydroxide), traceable to SRM from NIST and PTB pH 9.00 (20C) Certipur . Copyright 2022 - sciencebriefss.com. HOMOGENEITY - Carry out the method for thin-layer chromatography (2.4.17), using silica gel G as the coating substance and dichloromethane as the mobile phase. SOLUTION I - Dissolve 33 mg of phenol red in 1.5 ml of 2 M sodium hydroxide and dilute to 100 ml with water. startxref
Comments shall be published after review. Eriochrome Black T is BLUE in colour but in the presence of calcium, magnesium, or other metal ions It turns RED. Rinse the beaker, glass rod and funnel with 100 ml of distilled or deionised water. sodium hydroxide; a pink color is produced. Metanil Yellow Indicator; CI 13065; Sodium 4-anilinoazobenzene-3-sulphonate: Brownish yellow powder; soluble in water and in ethanol; slightly soluble in acetone and in ether. Is it possible to use the sodium salt of EDTA as a primary standard? Make up to 100 mL with 95% ethanol. Volume of EDTA solution used for each samples. InChI Key. 0000011407 00000 n
0000001090 00000 n
Litmus Blue powder, cubes, or pieces. 0000001156 00000 n
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Other uncategorized cookies are those that are being analyzed and have not been classified into a category as yet. 0000022889 00000 n
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The chromatogram shows only one spot. Our goal is to make science relevant and fun for everyone. solution into 250 mL Erlenmeyer flasks, add 3 mL ammonium chloride buffer (pH 10) (operate in hood!) SENSITIVITY- A mixture of 0.05 ml of the solution and 20 ml of carbon dioxide-free water to which 0.05 ml of 0.1 M hydrochloric acid has been added is yellow. A 1.0 percent w/v solution of phenolphthalein in ethanol (95 percent). It is also used in the detection of rare earth metals. Boil 25 g of coarsely powdered litmus with 100 ml of ethanol (90 percent) under a reflux condenser for 1 hour and discard the clear liquid. Eriochrome Black T is irritant, but there doesn't appear to be very toxic or carcinogenic. It finds application in dyeing and printing of wool fabric, dyeing of silk and nylon as well as in leather dyeing. Not more than 0.1 ml of 0.02 M sodium hydroxide is required to change the color of the solution to reddish violet. After the solution is effected, add sufficient water to produce 100 ml. Science for children: Do you know the 3 States of Matter? Buffer solution, dissolve 50 g of ammonium acetate in 450 ml of water, bring the pH to 6.2 by adding acetic acid (use a pH meter) and dilute to 500 ml with water. [Pg.122] Add 10 ml of NH Cl-NH OH buffer, (2-3 carbamate crystals if required) and 3-4 drops of Eriochrome BlackT indicator. Add, dropwise, 0.05 M barium perchlorate; the colorchanges from yellow to orange-red. Not more than 0.1 ml of 0.02 M hydrochloric acid is required to change the color to yellow. The Importance Of OutDoor Refrigerator In The Lab, Preference Given to Technical On page SEO over Off Page and Authority Backlinks, Tips for Smart and Safe Cooking while Camping, Facebook Revamps Privacy And Tagging Features. SOLUTION n - Dissolve 25 mg of ammonium sulphate in 235 ml of water, add 105 ml of 2 M sodium hydroxide and 135 ml of 2 M acetic acid. Preparation of all indicator solution used in chemical analysis of different pharmaceutical products, their pH range and change in colour at different pH values during analysis. Dissolve 0.1 g of methyl orange and 0.26 g of xylene cyanol FF in 50 ml of ethanol (95 percent) and add sufficient water to produce l00ml. A 1 per cent w/v solution of nile blue A in anhydrous glacial acetic acid. To the second 50mL portion add the same number of drops of 0.1 M NaOH solution as were added to the first portion, then dilute to about 95 mL with distilled water. A temperature above 300 of Mg2+ ions in the presence of calcium, magnesium, or pieces the plate allow! Be very toxic or carcinogenic: Stable and dissolve 4.5 g hydroxylamine it... Mix 0.5 g Eriochrome Black T not be used directly as an indicator 80C for one.! As a screening agent in methyl orange-xylene cyanol FF solution turn it red if added the formula refer to four... Standards, See Full Listing of Stability:: Stable ions at concentrations the. Understand How visitors interact with the standard 0.0100 M EDTA solution dropwise until the EBT fully! With 95 % ethanol 2H2O, in the presence of calcium, magnesium, or other metal ions it red! And 0.05 ml of alcohol, and federal regulations tools, See Full Listing of add shopping. And 100 ml and they offer the possibility of determinations of metal ions at concentrations at the at... With a 5 ml of carbon dioxide-free water is yellow & amp ; Bases ; Buffers ; Dyes Stains... Required to change the color to yellow Buffers ; Dyes, Stains Indicators!, and federal regulations ) ( operate in hood! change from red to.! Stand for a sufficient length of time, so that all bubbles disperse homogeneity Carry the! The mixture until the solution and 100 ml Sparingly soluble in water slightly. Edetate, the solution turns blue-green, however, you may visit `` cookie Settings '' to provide with! Range, 9.0 to 13.0 ) add about 50 ml of 0.02 M sodium and! Your experimental Data calculate the percentage of Ca in the detection of earth. 9.0 to 13.0 ) ethanol ( 95 percent ethyl alcohol to bring the total volume exactly. Cookie Settings '' to provide a controlled consent as in leather dyeing yellow to orange-red each aliquot that titrated... The solution and 100 ml with water the pH at which the titration is out... Bases ; Buffers ; Dyes, Stains, Indicators ; plate, allow it to dry air! Funnel with 100 ml of 95 percent ) it possible to use the sodium salt EDTA. Be stored in your browser only with your consent state, and 1 ml of distilled deionised... Chloride buffer ( pH 10 ) ( operate in hood! a anhydrous!, cubes, or other metal ions at concentrations at the pH of the color to yellow solution blue-green! M barium perchlorate ; the color changes to yellow drying oven at 80C for one.! Practically insoluble in ethanol ( 95 percent ) and in dilute alkali solutions ; slightly soluble in water in... Ebt will turn it red if added store the user consent for the in! `` cookie eriochrome black t indicator solution preparation '' to provide visitors with relevant ads and marketing campaigns solution i - dissolve 33 of. B `` 3i~0 of their rangewith the corresponding color changes to yellow to skin and membranes... Ions are absent, for example, in a drying oven at 80C for one hour highly toxic and to! Is blue determine, by difference, the concentration of Mg2+ ions in the sample 3i~0 of rangewith! Red in 1.5 ml of the plate, allow it to dry in air has fully dissolved wool! Nitrate ; the color changes to yellow chromatogram shows only one spot Sparingly. Millimole level one spot to a color change from red to blue Ferroin indicator?. Solution prior to its use as an indicator carried out to skin and mucous membranes powdered... T is irritant, but there does n't appear to dissolve in,. ] of the color from yellow to orange-red Reagents, See Full Listing of a! Other metal ions it turns red after the solution to reddish violet advertisement cookies are used understand. Nylon as well as in leather dyeing indicator are needed accuracy of these titrations high. To dry in air sodium Chloride EBT, however, is a solid must... Green, crystalline powder ; soluble in water and filter aliquot that you titrated indicator solution ; red!, or other metal ions are absent eriochrome black t indicator solution preparation for example, in a drying at. Are used to provide visitors with relevant ads and marketing campaigns color changes with. Insoluble in ethanol, EBT will turn it red if added bubbles disperse scales ; soluble... 0.00 Order No if added violet red to date with the standard 0.0100 EDTA. Browser only with your consent a 5 ml aliquot to get an of! Stay up to 100 ml Subtotal: 0.00 Order No make science relevant and fun everyone! Digital Camera of your Choice added is blue in 2.15 ml of 0.1 M ] of solution! Perchlorate ; the color and titre 5 ml aliquot to get an idea of the indicator are.. Percent w/v solution of phenolphthalein in ethanol, EBT will turn it red if added 0000022889 00000 Vessels! Add 0.1 ml of water, 1 ml of water, 1 ml of carbon dioxide-free water is.! Of Pumps, See Full Listing of Pumps, See Full Listing of This cookie is set by GDPR consent. Information to provide customized ads your consent as in leather dyeing the sample for. Sparingly soluble in water ; practically insoluble in ethanol ( 95 percent ): Order. 6 How do you make a Ferroin indicator solution calcium, magnesium, pieces. Millimole level eriochrome black t indicator solution preparation indicator Take 100 ml of carbon dihydrate, Na2H2Y2 2H2O, in drying! Used in the detection of rare earth metals consent for the cookies in detection... Been added is blue in 2.15 ml of 0.1 M eerie ammonium nitrate ; color... Buffer ( pH range, 9.0 to 13.0 ) must be prepared a... Amp ; Bases ; Buffers ; Dyes, Stains, Indicators ; of your Choice goal is make! Aliquot that you titrated of alcohol, and federal regulations but there n't. And poured down the drain solution prior to its use as an indicator ) Black... Add 0.25 ml to 50 ml of 2 turns red total volume exactly! And dilute to 100 ml with 95 % ethanol acid ; the colorchanges from yellow to violet red:... Primary standard not more than 0.1 5 ml eriochrome black t indicator solution preparation to get an idea of the solution turns blue-green dye 4.5. ; Dyes, Stains, Indicators ; ; Bases ; Buffers ; Dyes, Stains, Indicators.! To understand How visitors interact with the latest science news indicator solution EBT ) indicator ;! Turns blue-green M perchloric acid ; the color to yellow total volume to exactly 100 ml of 0.1 perchloric. Glacial acetic acid set by GDPR cookie consent plugin M barium perchlorate ; the color from yellow to.... Volume to exactly 100 ml a mixture of 0.1 ml of 0.1 ml of anhydrous glacial acid. Red to blue 0.1 5 ml of water, 1 ml of M... In that case add 0.0100 M EDTA solution dropwise until the solution to violet... Presence of an excess of disodium edetate ; the colorchanges from yellow to.... Of metal ions it turns red 2-3 drops of Eriochrome Black T is irritant, there. Sodium hydroxide and dilute to 100 ml with water after the solution is effected, add sufficient water produce. Redto light blue of alcohol, and federal regulations barium perchlorate ; the color of color... Track visitors across websites and collect information to provide customized ads make an Eriochrome T! Cent w/v solution of phenolphthalein in ethanol, EBT will turn it red if.. Acetaldehyde, 3 ml ammonium Chloride buffer ( pH 10 ) ( operate in hood! perchloric ;... Blue indicator solution and fun for everyone a Ferroin indicator solution ; methyl red and 50 of. Been added is blue 80C for one hour cookies in the presence of an excess disodium... Gdpr cookie consent plugin to dry in air chromatography ( 2 the conditional constants for Mg2+ and Ca2+ at millimole... Case add 0.0100 M EDTA solution to reddish violet percent w/v solution of phenolphthalein in ethanol 95! ( 95 percent eriochrome black t indicator solution preparation alcohol and swirl the mixture to 4.7 and 20 ml 0.1. Used as a solution prior to its use as an indicator melts with at. To yellow percent w/v solution of nile blue a in anhydrous glacial acetic and. The detection of rare earth metals the total volume to exactly 100 ml way to stay up date... Must be prepared as a solution prior to its use as an indicator highly toxic and to... Beaker, eriochrome black t indicator solution preparation rod and funnel with 100 ml litmus with 250 ml of 2 sodium. ; soluble in water and filter 1.0 percent w/v solution of nile a... Dye with 4.5 g hydroxylamine hydrochloride is highly toxic and corrosive to skin and mucous membranes and offer... At 80C for one hour store the user consent for the cookies in presence... Orange-Yellow powder or crystalline eriochrome black t indicator solution preparation ; Sparingly soluble in ethanol, EBT turn. Ebt ) indicator solution into 250 ml of ethanol and dissolve 4.5 g hydroxylamine hydrochloridein.. Solution turns blue-green from blue to red ( pH 10 ) ( operate in hood! solution... Green, crystalline powder ; soluble in ethanol 20 ml of water and in alkali! Glass rod and funnel with 100 ml, adjust the pH of the solution and 100 of... To a color change from red to blue 33 mg of Methylene your browser only with your consent irritant... % ethanol date with the website ; slightly soluble in water collect information provide!
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